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Magn Reson Med. 2012 Apr;67(4):1154-8. doi: 10.1002/mrm.23082. Epub 2011 Oct 18.

3D magnetic resonance microscopy of the ex vivo retina.

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1
University of Texas Health Science Center, San Antonio, TX, USA.

Abstract

3D-MR microscopy at 11.7T and 20 × 20 × 57 μm resolution was performed on formalin-fixed rat eyes with: (I) no contrast agent and (II) Gadodiamide (Omniscan(®) ) added to the fixative. Group I data showed generally poor contrast among layers. Group II data showed markedly better lamina-specific contrast with the nerve fiber + ganglion cell layer and inner nuclear layer being hypointense, and the inner plexiform, outer plexiform, outer nuclear layer, and the segments being hyperintense. The signal-to-noise ratio in group II was higher than group I, consistent with Gadodiamide acting as a T(1) -contrast agent. All major retinal layers were assigned and their thicknesses quantified with corroboration by histology. MR microscopy allows nondestructive examination of valuable specimens and could have applications in disease and in vivo.

PMID:
22009721
PMCID:
PMC3262931
DOI:
10.1002/mrm.23082
[Indexed for MEDLINE]
Free PMC Article
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