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Cancer. 2012 Apr 15;118(8):2106-16. doi: 10.1002/cncr.26488. Epub 2011 Aug 25.

CAPER-α alternative splicing regulates the expression of vascular endothelial growth factor₁₆₅ in Ewing sarcoma cells.

Author information

1
Division of Pediatrics, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA.

Abstract

BACKGROUND:

TC-71 Ewing sarcoma cells overexpress vascular endothelial growth factor (VEGF) with a shift from the 189 to the 165 isoform.

METHODS:

The effect of CAPER-α on the expression of the VEGF isoforms, tumor growth, and vessel density was analyzed after transfection of TC-71 cells with CAPER-α cDNA or siRNA.

RESULTS:

CAPER-α correlated inversely with the VEGF(165) /VEGF(189) mRNA ratio. Up-regulation of CAPER-α resulted in decreased tumor growth, tumor vessel density, and chemotactic activity of the cell's supernatant. CAPER-α expression was regulated by EWS/FLI-1 through a protein-protein interaction.

CONCLUSIONS:

Increased VEGF(165) expression is secondary to the down-regulation of CAPER-α by EWS/FLI-1. CAPER-α mediates alternative splicing and controls the shift from VEGF(189) to VEGF(165) .

PMID:
22009261
PMCID:
PMC3262942
DOI:
10.1002/cncr.26488
[Indexed for MEDLINE]
Free PMC Article
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