Format

Send to

Choose Destination
Development. 2011 Nov;138(22):4831-41. doi: 10.1242/dev.072587. Epub 2011 Oct 17.

Regeneration of the adult zebrafish brain from neurogenic radial glia-type progenitors.

Author information

1
Biotechnology Center and DFG-Research Center for Regenerative Therapies Dresden, Technische Universitat Dresden, Tatzberg 47/49, 01307 Dresden, Germany.

Abstract

Severe traumatic injury to the adult mammalian CNS leads to life-long loss of function. By contrast, several non-mammalian vertebrate species, including adult zebrafish, have a remarkable ability to regenerate injured organs, including the CNS. However, the cellular and molecular mechanisms that enable or prevent CNS regeneration are largely unknown. To study brain regeneration mechanisms in adult zebrafish, we developed a traumatic lesion assay, analyzed cellular reactions to injury and show that adult zebrafish can efficiently regenerate brain lesions and lack permanent glial scarring. Using Cre-loxP-based genetic lineage-tracing, we demonstrate that her4.1-positive ventricular radial glia progenitor cells react to injury, proliferate and generate neuroblasts that migrate to the lesion site. The newly generated neurons survive for more than 3 months, are decorated with synaptic contacts and express mature neuronal markers. Thus, regeneration after traumatic lesion of the adult zebrafish brain occurs efficiently from radial glia-type stem/progenitor cells.

PMID:
22007133
DOI:
10.1242/dev.072587
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center