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J Vis Exp. 2011 Oct 12;(56). pii: 2723. doi: 10.3791/2723.

Impulsive pressurization of neuronal cells for traumatic brain injury study.

Author information

1
Department of Engineering Mechanics, University of Nebraska-Lincoln, USA.

Abstract

A novel impulsive cell pressurization experiment has been developed using a Kolsky bar device to investigate blast-induced traumatic brain injury (TBI). We demonstrate in this video article how blast TBI-relevant impulsive pressurization is applied to the neuronal cells in vitro. This is achieved by using well-controlled pressure pulse created by a specialized Kolsky bar device, with complete pressure history within the cell pressurization chamber recorded. Pressurized neuronal cells are inspected immediately after pressurization, or further incubated to examine the long-term effects of impulsive pressurization on neurite/axonal outgrowth, neuronal gene expression, apoptosis, etc. We observed that impulsive pressurization at about 2 MPa induces distinct neurite loss relative to unpressurized cells. Our technique provides a novel method to investigate the molecular/cellular mechanisms of blast TBI, via impulsive pressurization of brain cells at well-controlled pressure magnitude and duration.

PMID:
22005926
PMCID:
PMC3227203
DOI:
10.3791/2723
[Indexed for MEDLINE]
Free PMC Article

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