Format

Send to

Choose Destination
See comment in PubMed Commons below
Planta Med. 2012 Jan;78(2):160-5. doi: 10.1055/s-0031-1280293. Epub 2011 Oct 14.

Isolation of apoptosis-inducing stilbenoids from four members of the Orchidaceae family.

Author information

1
Lead Discovery and Rapid Structure Elucidation Group, Sequoia Sciences, Inc., St. Louis, Missouri, USA. rwilliams@sequoiasciences.com

Abstract

High-throughput natural product research produced a suite of anticancer hits among several species of the Orchidaceae family (Oncidium microchilum, O. isthmi, and Myrmecophila humboldtii). A commercial Oncidium sp. was also examined as a convenient source of additional material. Isolation and structure elucidation led to the identification of fifteen stilbenoids including a new phenanthraquinone and two new dihydrostilbenes. NMR data for structure elucidation and dereplication were acquired utilizing a Bruker BioSpin TCI 1.7-mm MicroCryoProbe or a 5-μL CapNMR capillary microcoil. Several compounds inhibited proliferation of NCI-H460 and M14 cancer cell lines. All compounds were also examined for their ability to induce apoptosis. Apoptosis induction was determined by measuring caspase 3/7 activation and LDH release in a NCI-H460 cell line. Based on these results, a portion of the extract from a commercially available Oncidium sp. was chemically modified in an attempt to obtain additional phenanthraquinones.

PMID:
22002852
DOI:
10.1055/s-0031-1280293
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Georg Thieme Verlag Stuttgart, New York
    Loading ...
    Support Center