Format

Send to

Choose Destination
See comment in PubMed Commons below
Anal Bioanal Chem. 2011 Dec;401(10):3221-7. doi: 10.1007/s00216-011-5460-y. Epub 2011 Oct 15.

A standard additions method reduces inhibitor-induced bias in quantitative real-time PCR.

Author information

1
LGC Limited, Queens Road, Teddington, UK. s.ellison@lgc.co.uk

Abstract

A method of calibration for real-time quantitative polymerase chain reaction (qPCR) experiments based on the method of standard additions combined with non-linear curve fitting is described. The method is tested by comparing the results of a traditionally calibrated qPCR experiment with the standard additions experiment in the presence of 2 mM EDTA, a known inhibitor chosen to provide an unambiguous test of the principle by inducing an approximately twofold bias in apparent copy number calculated using traditional calibration. The standard additions method is shown to substantially reduce inhibitor-induced bias in quantitative real-time qPCR.

PMID:
22002559
DOI:
10.1007/s00216-011-5460-y
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer
    Loading ...
    Support Center