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Opt Lett. 2011 Oct 15;36(20):3987-9. doi: 10.1364/OL.36.003987.

Microscopy refocusing and dark-field imaging by using a simple LED array.

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1
Electrical Engineering, California Institute of Technology, Pasadena, California 91125, USA. gazheng@caltech.edu

Abstract

The condenser is one of the main components in most transmitted light compound microscopes. In this Letter, we show that such a condenser can be replaced by a programmable LED array to achieve greater imaging flexibility and functionality. Without mechanically scanning the sample or changing the microscope setup, the proposed approach can be used for dark-field imaging, bright-field imaging, microscopy sectioning, and digital refocusing. Images of a starfish embryo were acquired by using such an approach for demonstration.

PMID:
22002361
[Indexed for MEDLINE]
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