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Nat Methods. 2011 Oct 16;8(12):1044-6. doi: 10.1038/nmeth.1734.

Super-resolution 3D microscopy of live whole cells using structured illumination.

Author information

1
Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA. shaol@janelia.hhmi.org

Abstract

Three-dimensional (3D) structured-illumination microscopy (SIM) can double the lateral and axial resolution of a wide-field fluorescence microscope but has been too slow for live imaging. Here we apply 3D SIM to living samples and record whole cells at up to 5 s per volume for >50 time points with 120-nm lateral and 360-nm axial resolution. We demonstrate the technique by imaging microtubules in S2 cells and mitochondria in HeLa cells.

PMID:
22002026
DOI:
10.1038/nmeth.1734
[Indexed for MEDLINE]
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