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J Clin Microbiol. 2011 Dec;49(12):4225-30. doi: 10.1128/JCM.00334-11. Epub 2011 Oct 12.

Direct detection of Staphylococcus osteoarticular infections by use of Xpert MRSA/SA SSTI real-time PCR.

Author information

1
Laboratoire, Clinique de L'Union, Bd de Ratalens, 31240 St Jean, France. anne.dubouix@clinique-union.fr

Abstract

We evaluated the Xpert MRSA/SA SSTI real-time PCR assay (Cepheid, Sunnyvale, CA) directly on perioperative bone and joint samples. The sensitivity and specificity for detection of methicillin-susceptible Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA), and methicillin-resistant coagulase-negative Staphylococcus were, respectively, 100% and 98.3%, 100% and 100%, and 100% and 95.3%. The median total test turnaround time was 72 min for PCR versus 79 h for culture. Using these rapid results, appropriate antibiotic treatment could be rapidly initiated.

PMID:
21998420
PMCID:
PMC3232950
DOI:
10.1128/JCM.00334-11
[Indexed for MEDLINE]
Free PMC Article

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