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Methods Enzymol. 1990;181:400-21.

Genetic methods for study of trans-acting genes involved in processing of precursors to yeast cytoplasmic transfer RNAs.


All of the strategies to identify mutants defective in pre-tRNA processing have proved to be useful. However, in some of these strategies (such as inability to derepress a nonsense suppressor, correction of processing-defective substrates, and reverse genetics) only a single mutant has been isolated. This is not because the methods have proved to be inefficient, but rather because the field is still in infancy. Further work is needed to saturate the types of mutants found by each procedure. Undoubtedly, new strategies will be designed.

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