Hepatoprotective effect of prolactin and cysteine contained in perfusion and preservation solutions on porcine liver stored in simple hypothermia

Transplant Proc. 2011 Oct;43(8):2882-6. doi: 10.1016/j.transproceed.2011.08.064.

Abstract

Introduction: The aim of our study was to determine the results of histidine-tryptophan-ketoglutarate (HTK) solutions modified by the addition of the antioxidant cysteine (Cys), and of prolactin (PRL) on storage of isolated porcine livers.

Methods: We measured in the media of isolated livers stored for 24 hours in HTK (control group) or modified HTK+Cys (0.3 mmol/L)+PRL (3 IU/L study group) the amounts of aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), lactic acid as well as Ca (II), Mg (II), Na (I) and K (I) ions during a 30-minute perfusion after 24 hours of storage.

Results: All tested markers were released more slowly into HTK+Cys+PRL with less release of K(I) and Mg(II) and greater of Na(I) and Ca(II) ions.

Conclusions: Addition of the Cys and PRL to HTK positively affected 24-hour storage of isolated livers.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alanine Transaminase / metabolism
  • Animals
  • Aspartate Aminotransferases / metabolism
  • Cations / metabolism
  • Cold Ischemia
  • Cysteine / administration & dosage*
  • Female
  • Glucose / chemistry
  • In Vitro Techniques
  • L-Lactate Dehydrogenase / metabolism
  • Lactic Acid / metabolism
  • Liver / drug effects*
  • Liver / metabolism*
  • Liver Transplantation
  • Mannitol / chemistry
  • Organ Preservation / methods*
  • Organ Preservation Solutions / chemistry
  • Perfusion
  • Potassium Chloride / chemistry
  • Procaine / chemistry
  • Prolactin / administration & dosage*
  • Sus scrofa
  • Time Factors

Substances

  • Bretschneider cardioplegic solution
  • Cations
  • Organ Preservation Solutions
  • Lactic Acid
  • Mannitol
  • Procaine
  • Potassium Chloride
  • Prolactin
  • L-Lactate Dehydrogenase
  • Aspartate Aminotransferases
  • Alanine Transaminase
  • Glucose
  • Cysteine