Format

Send to

Choose Destination
Methods Mol Biol. 2012;799:405-19. doi: 10.1007/978-1-61779-346-2_22.

A DNA vaccine strategy for effective antibody induction to pathogen-derived antigens.

Author information

1
Division of Cancer Sciences, Genetic Vaccines Group, University of Southampton Medical School, Southampton, UK. J.Rice@soton.ac.uk

Abstract

DNA-based vaccines are currently being developed for treating a diversity of human diseases including cancers, autoimmune conditions, allergies, and microbial infections. In this chapter, we present a general protocol that can be used as a starting point for developing DNA vaccines to pathogen-derived antigens, using Neisseria meningitidis as an example. In addition, we describe a fusion gene-based vaccine protocol for increasing the potency of DNA vaccines that are based on poorly immunogenic antigens such as short pathogen-derived polypeptides. Finally, we provide a safe and effective protocol for delivery of DNA vaccines, based on intramuscular injection followed by electroporation.

PMID:
21993657
DOI:
10.1007/978-1-61779-346-2_22
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center