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PLoS One. 2011;6(10):e25708. doi: 10.1371/journal.pone.0025708. Epub 2011 Oct 3.

Effects of hormone agonists on Sf9 cells, proliferation and cell cycle arrest.

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1
Institut National de la Recherche Agronomique, UMR 1301 Interactions Biotiques et Santé Végétale, Centre National de la Recherche Scientifique, UMR 6243, Université de Nice Sophia Antipolis, Sophia-Antipolis, France.

Abstract

Methoxyfenozide and methoprene are two insecticides that mimic the action of the main hormones involved in the control of insect growth and development, 20-hydroxyecdysone and juvenile hormone. We investigated their effect on the Spodoptera frugiperda Sf9 cell line. Methoxyfenozide was more toxic than methoprene in cell viability tests and more potent in the inhibition of cellular proliferation. Cell growth arrest occurred in the G2/M phase after a methoprene treatment and more modestly in G1 after methoxyfenozide treatment. Microarray experiments and real-time quantitative PCR to follow the expression of nuclear receptors ultraspiracle and ecdysone receptor were performed to understand the molecular action of these hormone agonists. Twenty-six genes were differentially expressed after methoxyfenozide treatment and 55 genes after methoprene treatment with no gene in common between the two treatments. Our results suggest two different signalling pathways in Sf9 cells.

PMID:
21991338
PMCID:
PMC3185036
DOI:
10.1371/journal.pone.0025708
[Indexed for MEDLINE]
Free PMC Article
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