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J Infect Dis. 2011 Dec 1;204(11):1779-86. doi: 10.1093/infdis/jir639. Epub 2011 Oct 11.

Human cellular immune response against Giardia lamblia 5 years after acute giardiasis.

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1
Institute of Medicine, University of Bergen, Bergen, Norway. kurt.hanevik@med.uib.no

Abstract

BACKGROUND:

Clinical and epidemiological studies have suggested the development of acquired immunity in individuals previously infected with Giardia lamblia. However, there are no data on the long-term cellular immunity and genotype cross-reactivity. An outbreak of assemblage B giardiasis in a nonendemic area made it possible to evaluate the long-term cellular mediated immunity and its specificity toward the 2 Giardia assemblages known to infect humans.

METHODS:

Peripheral blood mononuclear cells from 19 individuals infected with Giardia assemblage B 5 years previously and from 10 uninfected controls were cultured with antigens from assemblage A and B Giardia trophozoites for 6 days. Cell-mediated immunity was measured by a (3)H-thymidine proliferation assay and flow cytometric analysis of activation markers HLA-DR, CD45RO, CD25, and CD26 in T-cell subsets.

RESULTS:

Proliferation responses were significantly elevated in the group previously exposed to Giardia for nearly all Giardia antigens tested. Individual responses toward Giardia trophozoite whole cell, cytosolic, and excretory-secretory antigens from both assemblages correlated well. Activation marker responses were mainly seen in CD4 T cells.

CONCLUSIONS:

G. lamblia infection induces long-term, albeit variable, cellular immune responses that are not assemblage specific and that are largely driven by CD4 T-cell activation.

PMID:
21990423
DOI:
10.1093/infdis/jir639
[Indexed for MEDLINE]
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