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J Cereb Blood Flow Metab. 2012 Jan;32(1):17-22. doi: 10.1038/jcbfm.2011.146. Epub 2011 Oct 12.

Enhanced SUMOylation and SENP-1 protein levels following oxygen and glucose deprivation in neurones.

Author information

1
MRC Centre for Synaptic Plasticity, School of Biochemistry, University of Bristol, University Walk, Bristol, UK. h.i.cimarosti@reading.ac.uk

Abstract

Here, we show that oxygen and glucose deprivation (OGD) causes increased small ubiquitin-like modifier (SUMO)-1 and SUMO-2/3 conjugation to substrate proteins in cultured hippocampal neurones. Surprisingly, the SUMO protease SENP-1, which removes SUMO from conjugated proteins, was also increased by OGD, suggesting that the neuronal response to OGD involves a complex interplay between SUMOylation and deSUMOylation. Importantly, decreasing global SUMOylation in cultured hippocampal neurones by overexpression of the catalytic domain of SENP-1 increased neuronal vulnerability to OGD-induced cell death. Taken together, these results suggest a neuroprotective role for neuronal SUMOylation after OGD.

PMID:
21989481
PMCID:
PMC3308141
DOI:
10.1038/jcbfm.2011.146
[Indexed for MEDLINE]
Free PMC Article

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