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J Control Release. 2012 Mar 28;158(3):433-42. doi: 10.1016/j.jconrel.2011.09.078. Epub 2011 Sep 24.

High-content screening of peptide-based non-viral gene delivery systems.

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Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Faculty of science, University of Utrecht, Universiteitsweg 99, 3584 CG Utrecht, The Netherlands.


High-content screening (HCS) uses high-capacity automated fluorescence imaging for the quantitative analysis of single cells and cell populations. Here, we developed an HCS assay for rapid screening of non-viral gene delivery systems as exemplified by the screening of a small library of peptide-based transfectants. These peptides were simultaneously screened for transfection efficiency, cytotoxicity, induction of cell permeability and the capacity to transfect non-dividing cells. We demonstrated that HCS is a valuable extension to the already existing screening methods for the in vitro evaluation of non-viral gene delivery systems with the added value that multiple parameters can be screened in parallel thereby obtaining more information from a single screening event, which will accelerate the development of novel gene delivery systems.

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