Format

Send to

Choose Destination
See comment in PubMed Commons below
Phys Rev Lett. 2011 Sep 2;107(10):108102. Epub 2011 Sep 1.

Underwound DNA under tension: structure, elasticity, and sequence-dependent behaviors.

Author information

1
Department of Physics, Laboratory of Atomic and Solid State Physics, Cornell University, Ithaca, New York 14853, USA.

Abstract

DNA melting under torsion plays an important role in a wide variety of cellular processes. In the present Letter, we have investigated DNA melting at the single-molecule level using an angular optical trap. By directly measuring force, extension, torque, and angle of DNA, we determined the structural and elastic parameters of torsionally melted DNA. Our data reveal that under moderate forces, the melted DNA assumes a left-handed structure as opposed to an open bubble conformation and is highly torsionally compliant. We have also discovered that at low forces melted DNA properties are highly dependent on DNA sequence. These results provide a more comprehensive picture of the global DNA force-torque phase diagram.

PMID:
21981534
PMCID:
PMC3201814
DOI:
10.1103/PhysRevLett.107.108102
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for American Physical Society Icon for PubMed Central
    Loading ...
    Support Center