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Commun Integr Biol. 2011 Jul;4(4):409-12. doi: 10.4161/cib.4.4.15250. Epub 2011 Jul 1.

Aurora A kinase activity is required for localization of TACC3/ch-TOG/clathrin inter-microtubule bridges.

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1
The Physiological Laboratory; University of Liverpool; Liverpool, UK.

Abstract

Accurate chromosome segregation during mitosis is achieved by the kinetochore fibers (K-fibers) of the spindle apparatus. These fibers are bundles of microtubules (MTs) connected by non-motor bridges. We recently identified a TACC3/ch-TOG/clathrin complex that constitutes the shortest class of inter-MT bridge in K-fibers. TACC3 anchors the complex to MTs and this is dependent on phosphorylation by Aurora A kinase. Here we show that inhibition of Aurora A kinase using MLN8237 results in (1) loss of clathrin and TACC3 from spindles, (2) destabilization of K-fibers and (3) loss of inter-MT bridges. These results are similar to those in cells depleted of clathrin or TACC3; suggesting that TACC3/ch-TOG/clathrin bridges are the major class of bridge that is regulated by this kinase.

KEYWORDS:

MLN8054; MLN8237; TACC3; aurora A kinase; cancer; ch-TOG; clathrin; inter-microtubule bridge; microtubule; mitotic spindle

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