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Nat Methods. 2011 Oct 2;8(11):933-5. doi: 10.1038/nmeth.1716.

Gas-phase purification enables accurate, multiplexed proteome quantification with isobaric tagging.

Author information

1
Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.

Abstract

We describe a mass spectrometry method, QuantMode, which improves accuracy of isobaric tag-based quantification by alleviating the pervasive problem of precursor interference, simultaneous isolation and fragmentation of impurities, through gas-phase purification. QuantMode analysis of a yeast sample 'contaminated' with interfering human peptides showed substantially improved quantitative accuracy compared to a standard scan, with a small loss of spectral identifications. This technique enables large-scale, multiplexed quantitative proteomics using isobaric tagging.

PMID:
21963608
PMCID:
PMC3205195
DOI:
10.1038/nmeth.1716
[Indexed for MEDLINE]
Free PMC Article

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