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Nat Methods. 2011 Oct 2;8(11):937-40. doi: 10.1038/nmeth.1714.

MS3 eliminates ratio distortion in isobaric multiplexed quantitative proteomics.

Author information

1
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA.

Abstract

Quantitative mass spectrometry-based proteomics is highly versatile but not easily multiplexed. Isobaric labeling strategies allow mass spectrometry-based multiplexed proteome quantification; however, ratio distortion owing to protein quantification interference is a common effect. We present a two-proteome model (mixture of human and yeast proteins) in a sixplex isobaric labeling system to fully document the interference effect, and we report that applying triple-stage mass spectrometry (MS3) almost completely eliminates interference.

PMID:
21963607
PMCID:
PMC3205343
DOI:
10.1038/nmeth.1714
[Indexed for MEDLINE]
Free PMC Article

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