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Clin Biochem. 2011 Dec;44(17-18):1445-50. doi: 10.1016/j.clinbiochem.2011.09.010. Epub 2011 Sep 21.

The stability of markers in dried-blood spots for recommended newborn screening disorders in the United States.

Author information

1
Centers for Disease Control and Prevention, 4770 Buford Highway NE, Atlanta, GA 30341, USA. BAdam@cdc.gov

Abstract

OBJECTIVE:

We aimed to measure separately the contributions of heat and humidity to changes in levels of 34 markers of inborn disorders in dried-blood-spot (DBS) samples.

DESIGN AND METHODS:

We stored paired sets of DBSs at 37°C for predetermined intervals in low-humidity and high-humidity environments. Marker levels of all samples in each complete sample set were measured in a single analytic run.

RESULTS:

During the 30 ± 5 day studies, galactose-1-phosphate uridyltransferase and biotinidase lost almost 65% of initial activities in low-humidity storage; most of the degradation in 27 other markers was attributable to adverse effects of high-humidity storage; seven markers in DBSs stored at high humidity lost more than 90% of initial levels by the end of the study and 4 of the 7 lost more than 50% of initial levels within the first week of storage.

CONCLUSIONS:

Minimizing both humidity and temperature in DBS transportation and storage environments is essential to maintaining sample integrity.

PMID:
21963384
PMCID:
PMC4557772
DOI:
10.1016/j.clinbiochem.2011.09.010
[Indexed for MEDLINE]
Free PMC Article

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