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Vet Res. 2011 Sep 30;42:104. doi: 10.1186/1297-9716-42-104.

PRNP genetic variability and molecular typing of natural goat scrapie isolates in a high number of infected flocks.

Author information

1
National Agricultural Research Foundation, Veterinary Research Institute, Thessaloniki, Greece. eirfrag@gmail.com.

Abstract

One hundred and four scrapie positive and 77 negative goats from 34 Greek mixed flocks were analysed by prion protein gene sequencing and 17 caprine scrapie isolates from 11 flocks were submitted to molecular isolate typing. For the first time, the protective S146 variant was reported in Greece, while the protective K222 variant was detected in negative but also in five scrapie positive goats from heavily infected flocks. By immunoblotting six isolates, including two goat flockmates carrying the K222 variant, showed molecular features slightly different from all other Greek and Italian isolates co-analysed, possibly suggesting the presence of different scrapie strains in Greece.

PMID:
21961834
PMCID:
PMC3190342
DOI:
10.1186/1297-9716-42-104
[Indexed for MEDLINE]
Free PMC Article

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