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Front Neural Circuits. 2010 Aug 26;4:100. doi: 10.3389/fncir.2010.00100. eCollection 2010.

Ephus: multipurpose data acquisition software for neuroscience experiments.

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Department of Physiology, Feinberg School of Medicine, Northwestern University Chicago, IL, USA.


Physiological measurements in neuroscience experiments often involve complex stimulus paradigms and multiple data channels. Ephus ( is an open-source software package designed for general-purpose data acquisition and instrument control. Ephus operates as a collection of modular programs, including an ephys program for standard whole-cell recording with single or multiple electrodes in typical electrophysiological experiments, and a mapper program for synaptic circuit mapping experiments involving laser scanning photostimulation based on glutamate uncaging or channelrhodopsin-2 excitation. Custom user functions allow user-extensibility at multiple levels, including on-line analysis and closed-loop experiments, where experimental parameters can be changed based on recently acquired data, such as during in vivo behavioral experiments. Ephus is compatible with a variety of data acquisition and imaging hardware. This paper describes the main features and modules of Ephus and their use in representative experimental applications.


data acquisition; electrophysiology; imaging; mapping; software

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