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Cell Oncol (Dordr). 2012 Feb;35(1):29-41. doi: 10.1007/s13402-011-0059-z. Epub 2011 Sep 30.

MicroRNA cloning and sequencing in osteosarcoma cell lines: differential role of miR-93.

Author information

1
Center for Molecular and Translational Oncology, Department of Genetics, Microbiology, Antropology and Evolution, University of Parma, Parma, Italy.

Abstract

BACKGROUND:

Studies show that abnormalities in non-coding genes can contribute to carcinogenesis; microRNA levels may modulate cancer growth and metastatic diffusion.

METHOD:

MicroRNA libraries were built and sequenced from two osteosarcoma cell lines (MG-63 and 143B), which differ in proliferation and transmigration. By cloning and transfection, miR-93, expressed in both cell lines, was then investigated for its involvement in osteosarcoma progression.

RESULTS:

Six of the 19 miRNA identified were expressed in both cell lines with higher expression levels of miR-93 in 143B and in primary osteosarcoma cultures compared to normal osteoblasts. Interestingly, levels of miR-93 were significantly higher in metastases from osteosarcoma than in paired primary tumours. When 143B and MG-63 were transfected with miR-93, clones appeared to respond differently to microRNA overexpression. Ectopic expression of miR-93 more significantly increased cell proliferation and invasivity in 143B than in MG-63 clones. Furthermore, increased mRNA and protein levels of E2F1, one of the potential miR-93 targets, were seen in osteosarcoma cellular clones and its involvement in 143B cell proliferation was confirmed by E2F1 silencing.

CONCLUSION:

Although further studies are needed to evaluate miRNA involvement in osteosarcoma progression, miR-93 overexpression seems to play an important role in osteosarcoma cell growth and invasion.

PMID:
21959981
DOI:
10.1007/s13402-011-0059-z
[Indexed for MEDLINE]

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