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Mitochondrion. 2012 Mar;12(2):213-9. doi: 10.1016/j.mito.2011.09.001. Epub 2011 Sep 17.

miR-181 targets multiple Bcl-2 family members and influences apoptosis and mitochondrial function in astrocytes.

Author information

1
Department of Anesthesia, Stanford University School of Medicine, Stanford, CA 94305, USA. ybouyang@stanford.edu

Abstract

Mitochondria are central to the execution of apoptosis, and the Bcl-2 protein family of pro- and anti-apoptotic proteins interacts with mitochondria to regulate apoptosis. Using bioinformatics we predicted that miR-181, a microRNA expressed in brain, could target the 3'UTRs of Bcl-2 family members Bcl-2-L11/Bim, Mcl-1, and Bcl-2. Using the luciferase reporter assay we confirmed these targets. We used mimic and inhibitor to alter miR-181a levels in primary astrocyte cultures and found miR-181a reduction was associated with increased Bcl-2 and Mcl-1 protein levels. Decreased miR-181a levels reduced glucose deprivation induced apoptosis, mitochondrial dysfunction, and loss of mitochondrial membrane potential in astrocytes.

PMID:
21958558
PMCID:
PMC3250561
DOI:
10.1016/j.mito.2011.09.001
[Indexed for MEDLINE]
Free PMC Article

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