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Microb Biotechnol. 2012 Jan;5(1):129-34. doi: 10.1111/j.1751-7915.2011.00304.x. Epub 2011 Sep 29.

Intracellular monitoring of target protein production in Staphylococcus aureus by peptide tag-induced reporter fluorescence.

Author information

1
Lehrbereich Mikrobielle Genetik, Waldhäuser Str. 70/8, Eberhard Karls Universität Tübingen, Germany.

Abstract

An intracellular approach for monitoring protein production in Staphylococcus aureus is described. mCherry, fused to the dodecapeptide Tip, was capable of inducing tetracycline repressor (TetR). Time- and concentration-dependent production of mCherry could be correlated to TetR-controlled GFPmut2 activity. This approach can potentially be extended to native S. aureus proteins.

PMID:
21958360
PMCID:
PMC3815279
DOI:
10.1111/j.1751-7915.2011.00304.x
[Indexed for MEDLINE]
Free PMC Article

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