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J Am Soc Mass Spectrom. 2011 Jun;22(6):1022-31. doi: 10.1007/s13361-011-0121-0. Epub 2011 Apr 9.

High-speed MALDI-TOF imaging mass spectrometry: rapid ion image acquisition and considerations for next generation instrumentation.

Author information

1
Mass Spectrometry Research Center, Vanderbilt University Medical Center, Nashville, TN, USA.

Abstract

A prototype matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometer has been used for high-speed ion image acquisition. The instrument incorporates a Nd:YLF solid state laser capable of pulse repetition rates up to 5 kHz and continuous laser raster sampling for high-throughput data collection. Lipid ion images of a sagittal rat brain tissue section were collected in 10 min with an effective acquisition rate of roughly 30 pixels/s. These results represent more than a 10-fold increase in throughput compared with current commercially available instrumentation. Experiments aimed at improving conditions for continuous laser raster sampling for imaging are reported, highlighting proper laser repetition rates and stage velocities to avoid signal degradation from significant oversampling. As new high spatial resolution and large sample area applications present themselves, the development of high-speed microprobe MALDI imaging mass spectrometry is essential to meet the needs of those seeking new technologies for rapid molecular imaging.

PMID:
21953043
PMCID:
PMC3514015
DOI:
10.1007/s13361-011-0121-0
[Indexed for MEDLINE]
Free PMC Article

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