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J Am Soc Mass Spectrom. 2011 Oct;22(10):1699-706. doi: 10.1007/s13361-011-0202-0. Epub 2011 Jul 16.

A new ion source and procedures for atmospheric pressure-electron capture dissociation of peptides.

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1
Department of Chemistry, University of British Columbia, Vancouver, BC, V6T 1Z1, Canada. robb@chem.ubc.ca

Abstract

We introduce a new atmospheric pressure-electron capture dissociation (AP-ECD) source in which conventional nanospray emitters are coupled with the source block and photoionization lamp of a PhotoSpray APPI source. We also introduce procedures for data collection and processing, aimed at maximizing the signal-to-background ratio of ECD products. Representative data from Substance P are presented to demonstrate the performance of the technique. Further, we demonstrate the effects of two important experimental variables, source temperature and vacuum-interface declustering potential (DP), on the method. Last, we show that even when a high source temperature is used to maximize efficiency, AP-ECD fragments of a model phosphorylated peptide retain the modification.

PMID:
21952883
DOI:
10.1007/s13361-011-0202-0
[Indexed for MEDLINE]
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