Send to

Choose Destination
See comment in PubMed Commons below
J Am Soc Mass Spectrom. 2011 Oct;22(10):1699-706. doi: 10.1007/s13361-011-0202-0. Epub 2011 Jul 16.

A new ion source and procedures for atmospheric pressure-electron capture dissociation of peptides.

Author information

Department of Chemistry, University of British Columbia, Vancouver, BC, V6T 1Z1, Canada.


We introduce a new atmospheric pressure-electron capture dissociation (AP-ECD) source in which conventional nanospray emitters are coupled with the source block and photoionization lamp of a PhotoSpray APPI source. We also introduce procedures for data collection and processing, aimed at maximizing the signal-to-background ratio of ECD products. Representative data from Substance P are presented to demonstrate the performance of the technique. Further, we demonstrate the effects of two important experimental variables, source temperature and vacuum-interface declustering potential (DP), on the method. Last, we show that even when a high source temperature is used to maximize efficiency, AP-ECD fragments of a model phosphorylated peptide retain the modification.

[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons


    Supplemental Content

    Full text links

    Icon for Springer
    Loading ...
    Support Center