Format

Send to

Choose Destination
See comment in PubMed Commons below
Age (Dordr). 2012 Dec;34(6):1317-40. doi: 10.1007/s11357-011-9306-5. Epub 2011 Sep 23.

The role of DNA exonucleases in protecting genome stability and their impact on ageing.

Author information

1
Department of Biochemistry, University of Oxford, UK. penelope.mason@bioch.ox.ac.uk

Abstract

Exonucleases are key enzymes involved in many aspects of cellular metabolism and maintenance and are essential to genome stability, acting to cleave DNA from free ends. Exonucleases can act as proof-readers during DNA polymerisation in DNA replication, to remove unusual DNA structures that arise from problems with DNA replication fork progression, and they can be directly involved in repairing damaged DNA. Several exonucleases have been recently discovered, with potentially critical roles in genome stability and ageing. Here we discuss how both intrinsic and extrinsic exonuclease activities contribute to the fidelity of DNA polymerases in DNA replication. The action of exonucleases in processing DNA intermediates during normal and aberrant DNA replication is then assessed, as is the importance of exonucleases in repair of double-strand breaks and interstrand crosslinks. Finally we examine how exonucleases are involved in maintenance of mitochondrial genome stability. Throughout the review, we assess how nuclease mutation or loss predisposes to a range of clinical diseases and particularly ageing.

PMID:
21948156
PMCID:
PMC3528374
DOI:
10.1007/s11357-011-9306-5
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer Icon for PubMed Central
    Loading ...
    Support Center