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J Biomol NMR. 2011 Sep;51(1-2):199-207. doi: 10.1007/s10858-011-9554-9. Epub 2011 Sep 27.

Origin and removal of mixed-phase artifacts in gradient sensitivity enhanced heteronuclear single quantum correlation spectra.

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1
Department of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands. f.a.a.mulder@rug.nl

Abstract

Here we describe phasing anomalies observed in gradient sensitivity enhanced (15)N-(1)H HSQC spectra, and analyze their origin. It is shown that, as a result of (15)N off-resonance effects, dispersive contributions to the (1)H signal become detectable, and lead to (15)N-offset dependent phase errors. Strategies that effectively suppress these artifacts are presented.

PMID:
21947928
PMCID:
PMC3180630
DOI:
10.1007/s10858-011-9554-9
[Indexed for MEDLINE]
Free PMC Article
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