Format

Send to

Choose Destination
Biochem Biophys Res Commun. 2011 Oct 14;414(1):165-9. doi: 10.1016/j.bbrc.2011.09.048. Epub 2011 Sep 17.

Nandrolone reduces activation of Notch signaling in denervated muscle associated with increased Numb expression.

Author information

1
Center of Excellence for Medical Consequences of Spinal Cord Injury, James J. Peter VA Medical Center, Bronx, NY 10468, USA.

Abstract

Nandrolone, an anabolic steroid, slows denervation-atrophy in rat muscle. The molecular mechanisms responsible for this effect are not well understood. Androgens and anabolic steroids activate Notch signaling in animal models of aging and thereby mitigate sarcopenia. To explore the molecular mechanisms by which nandrolone prevents denervation-atrophy, we investigated the effects of nandrolone on Notch signaling in denervated rat gastrocnemius muscle. Denervation significantly increased Notch activity reflected by elevated levels of nuclear Notch intracellular domain (NICD) and expression of Hey1 (a Notch target gene). Activation was greatest at 7 and 35 days after denervation but remained present at 56 days after denervation. Activation of Notch in denervated muscle was prevented by nandrolone associated with upregulated expression of Numb mRNA and protein. These data demonstrate that denervation activates Notch signaling, and that nandrolone abrogates this response associated with increased expression of Numb, suggesting a potential mechanism by which nandrolone reduces denervation-atrophy.

PMID:
21945932
DOI:
10.1016/j.bbrc.2011.09.048
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center