Format

Send to

Choose Destination
J Biotechnol. 2011 Dec 10;156(3):211-3. doi: 10.1016/j.jbiotec.2011.09.013. Epub 2011 Sep 17.

Using Vaccinia's innate ability to introduce DNA into mammalian cells for production of recombinant proteins.

Author information

1
Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, Institut de Biologie Moléculaire et Cellulaire, 15, rue René Descartes, F-67084 Strasbourg, France. jester_brian@hotmail.com

Abstract

Production of recombinant protein in mammalian cells is time-consuming, labor-intensive and costly. While seeking to overcome these limitations, we discovered that Vaccinia virus has the innate ability to transfer exogenous plasmid DNA into mammalian cells during the infection process. Parameters influencing the efficiency of this event were characterized and a quick, simple and inexpensive way to produce eukaryotic proteins was established.

PMID:
21945587
DOI:
10.1016/j.jbiotec.2011.09.013
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center