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J Virol Methods. 2011 Dec;178(1-2):137-42. doi: 10.1016/j.jviromet.2011.09.003. Epub 2011 Sep 17.

Inhibitory effects of silver nanoparticles on H1N1 influenza A virus in vitro.

Author information

1
Department of Microbiology, Medical College, Dalian University, Dalian 116622, Liaoning, China.

Abstract

Silver nanoparticles have demonstrated efficient inhibitory activities against human immunodeficiency virus (HIV) and hepatitis B virus (HBV). However, the effects of silver nanoparticles against H1N1 influenza A virus remain unexplored. In this study, the interaction of silver nanoparticles with H1N1 influenza A virus was investigated. Silver nanoparticles with mean particle diameters of 10nm were prepared for the hemagglutination inhibition test, the embryo inoculation assay, and the Mosmann-based 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, where these tests were used to determine the inhibitory activity of silver nanoparticles on H1N1 influenza A virus. MDCK cells were used as the infection model. Electron microscopy analysis and flow cytometry assay were used to determine whether silver nanoparticles could reduce H1N1 influenza A virus-induced apoptosis in MDCK cells. This study demonstrates that silver nanoparticles have anti-H1N1 influenza A virus activities. The inhibitory effects of silver nanoparticles on influenza A virus may be a novel clinical strategy for the prevention of influenza virus infection during the early dissemination stage of the virus.

PMID:
21945220
DOI:
10.1016/j.jviromet.2011.09.003
[Indexed for MEDLINE]

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