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Curr Opin Neurobiol. 2012 Feb;22(1):94-100. doi: 10.1016/j.conb.2011.08.004. Epub 2011 Sep 22.

The gain in brain: novel imaging techniques and multiplexed proteomic imaging of brain tissue ultrastructure.

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1
Stanford University School of Medicine, Department of Molecular and Cellular Physiology, Stanford, CA 94305, USA. kmicheva@stanford.edu

Abstract

The rapid accumulation of neuroproteomics data in recent years has prompted the emergence of novel antibody-based imaging methods that aim to understand the anatomical and functional context of the multitude of identified proteins. The pioneering field of ultrastructural multiplexed proteomic imaging now includes a number of high resolution methods, such as array tomography, stimulated emission depletion microscopy, stochastic optical reconstruction microscopy and automated transmission electron microscopy, which allow a detailed molecular characterization of individual synapses and subsynaptic structures within brain tissues for the first time. While all of these methods still face considerable limitations, a combined complementary approach building on the respective strengths of each method is possible and will enable fascinating research into the proteomic diversity of the nervous system.

PMID:
21944260
PMCID:
PMC3265692
DOI:
10.1016/j.conb.2011.08.004
[Indexed for MEDLINE]
Free PMC Article
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