Format

Send to

Choose Destination
See comment in PubMed Commons below
Neuron. 2011 Sep 22;71(6):1043-57. doi: 10.1016/j.neuron.2011.07.009. Epub 2011 Sep 21.

Axon regeneration pathways identified by systematic genetic screening in C. elegans.

Author information

1
Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA.

Abstract

The mechanisms underlying the ability of axons to regrow after injury remain poorly explored at the molecular genetic level. We used a laser injury model in Caenorhabditis elegans mechanosensory neurons to screen 654 conserved genes for regulators of axonal regrowth. We uncover several functional clusters of genes that promote or repress regrowth, including genes classically known to affect axon guidance, membrane excitability, neurotransmission, and synaptic vesicle endocytosis. The conserved Arf Guanine nucleotide Exchange Factor (GEF), EFA-6, acts as an intrinsic inhibitor of regrowth. By combining genetics and in vivo imaging, we show that EFA-6 inhibits regrowth via microtubule dynamics, independent of its Arf GEF activity. Among newly identified regrowth inhibitors, only loss of function in EFA-6 partially bypasses the requirement for DLK-1 kinase. Identification of these pathways significantly expands our understanding of the genetic basis of axonal injury responses and repair.

PMID:
21943602
PMCID:
PMC3183436
DOI:
10.1016/j.neuron.2011.07.009
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Support Center