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Plasmid. 1990 Mar;23(2):159-62.

A yeast-Escherichia coli shuttle vector containing the M13 origin of replication.

Author information

1
Department of Chemistry, University of North Carolina, Chapel Hill 27599-3290.

Abstract

A yeast-Escherichia coli shuttle vector containing the M13 origin of replication has been constructed. This vector allows selection and replication in both Saccharomyces cerevisiae and E. coli, as well as single-stranded packaging from E. coli upon infection with a helper phage. The presence of a polylinker with various unique restriction sites facilitates the cloning of desired genes.

PMID:
2194231
DOI:
10.1016/0147-619x(90)90036-c
[Indexed for MEDLINE]

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