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J Am Chem Soc. 2011 Nov 2;133(43):17103-5. doi: 10.1021/ja205137d. Epub 2011 Oct 7.

A chemical reporter for protein AMPylation.

Author information

1
The Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, New York 10065, USA.

Abstract

Protein AMPylation is an emerging post-translational modification, which plays key roles in bacterial pathogenesis and cell biology. Enzymes with AMPylation activity, referred to as AMPylators, have been identified in several bacterial pathogens and eukaryotes. To facilitate the study of this unique modification, we developed an alkynyl chemical reporter for detection and identification of protein AMPylation substrates. Covalent functionalization of AMPylation substrates with the alkynyl reporter in lieu of adenylyl 5'-monophosphate (AMP) allows their subsequent bioorthogonal ligation with azide-fluorescent dyes or affinity enrichment tags. We show that this chemical reporter is transferred by a range of AMPylators onto their cognate protein substrates and allows rapid detection and identification of AMPylated substrates.

PMID:
21942216
PMCID:
PMC3246509
DOI:
10.1021/ja205137d
[Indexed for MEDLINE]
Free PMC Article

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