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EMBO Rep. 2011 Oct 28;12(11):1153-9. doi: 10.1038/embor.2011.176.

miRNA screening reveals a new miRNA family stimulating iPS cell generation via regulation of Meox2.

Author information

1
REBIRTH-Group Reprogramming, Hannover Medical School, 30625 Hannover, Germany.

Abstract

Induced pluripotent stem cells (iPSCs) can be generated by overexpression of Oct4, Sox2 and Klf4 in murine fibroblasts. By conducting a microRNA (miRNA) library screen, we identified a set of miRNAs critically regulating iPSC formation. We revealed a new miRNA family (miR-130/301/721) as an important regulator of iPSC induction by targeting the homeobox transcription factor Meox2 (also known as Gax). Meox2-specific silencing mimicked the effects of this miRNA family on reprogramming. Mechanistically, miRNA-resistant Meox2 overexpression abrogated effects of miR-130/301/721 on reprogramming. In conclusion, the miRNA family miR-130/301/721 enhances iPSC generation via repression of Meox2.

PMID:
21941297
PMCID:
PMC3207101
DOI:
10.1038/embor.2011.176
[Indexed for MEDLINE]
Free PMC Article

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