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Methods Mol Biol. 2012;786:291-314. doi: 10.1007/978-1-61779-292-2_18.

Using cisTargetX to predict transcriptional targets and networks in Drosophila.

Author information

1
TAGC Inserm U928 and Université de la Mediterranée, Marseille, France.

Abstract

Gene expression regulation is a fundamental biological process leading to complete organism development by controlling processes like cell type specification and differentiation. The accuracy of this process is -governed by transcription factors (TFs) acting within a complex gene regulatory network. CisTargetX has been developed to enable a user to predict TFs, enhancers, and target genes involved in the regulation of co-expressed genes. It uses a strategy that incorporates the genome-wide prediction of clusters of transcription factor binding sites (TFBSs), starting from a large, unbiased collection of position weight matrices (PWMs) and uses comparative genomics criteria to filter potential TFBS. We describe in this chapter, step-by-step, how to use cisTargetX starting from a set of genes or TF(s) to predict transcriptional targets with their putative binding sites and networks in Drosophila. Next, we illustrate this approach on a particular developmental system, namely, sensory organ development, and identify relevant TFs, DNA regions regulating gene expression, and TF/target gene interactions. CisTargetX is available at http://med.kuleuven.be/lcb/cisTargetX .

PMID:
21938634
DOI:
10.1007/978-1-61779-292-2_18
[Indexed for MEDLINE]

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