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Methods Mol Biol. 2012;786:115-30. doi: 10.1007/978-1-61779-292-2_7.

Detecting protein-protein interactions with the Split-Ubiquitin sensor.

Author information

1
Department of Biology, Institute of Molecular Genetics and Cell Biology, Ulm University, Ulm, Germany.

Abstract

A detailed understanding of a cellular process requires the knowledge about the interactions between its protein constituents. The Split-Ubiquitin technique allows to monitor and detect interactions of very diverse proteins, including transcription factors and membrane-associated proteins. The technique is based on unique features of ubiquitin, the enzymes of the ubiquitin pathway, and the reconstitution of a native-like ubiquitin from its N- and C-terminal fragments. Using Ura3p as a reporter for the reconstitution of the ubiquitin fragments, methods are presented that enable to screen in yeast for interaction partners of a given protein with either a randomly generated expression library or a defined but more limited array of protein fusions.

PMID:
21938623
DOI:
10.1007/978-1-61779-292-2_7
[Indexed for MEDLINE]

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