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J Lab Physicians. 2009 Jul;1(2):69-72. doi: 10.4103/0974-2727.59703.

Optimum Time to Detection of Bacteria and Yeast Species with BACTEC 9120 Culture System from Blood and Sterile Body Fluids.

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1
Department of Microbiology, SMS Medical College, Jaipur, India.

Abstract

The culture results of 4,807 blood and 383 sterile body fluid specimens received in our laboratory during a 54-month period, were analyzed to determine the time required for culture to become positive, time at which a culture could safely be considered negative, and the spectrum of isolated organisms. The specimens were processed by automated BACTEC 9120 culture system. A total of 1,677 clinically significant microorganisms were isolated. Gram positive and negative bacterial isolation rates were found to be 62.55% and 32.20%, respectively. Yeasts were recovered in 5.24%. False positivity rate was 1.5%. Clinically significant isolates recovered on day four and five were 97.81% and 99.88%, respectively. At day five, the sensitivity was 99.94% and negative predictive value 99.96%. Our data support a five-day incubation protocol for recovery of all clinically significant organisms with sensitivity reduced by 0.06%, when compared with a six-day protocol.

KEYWORDS:

Automated blood culture system; BACTEC; time to detection

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