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Cell Immunol. 2011;271(2):329-34. doi: 10.1016/j.cellimm.2011.07.011. Epub 2011 Aug 25.

Characterization of polysaccharide from Astragalus radix as the macrophage stimulator.

Author information

1
Department of Genetics and Biochemistry, School of Medicine, Zhejiang University, Hangzhou, People's Republic of China. zhaoluhang@263.net

Abstract

Astragalus polysaccharide (APS) was obtained by hot water extraction, alcohol precipitation, gel-permeation chromatography and ultrafiltration. Fluorescence material 2-aminoacridone (2-AMAC) labeled APS bind to macrophage in a time- dependent manner and the binding can be remarkably inhibited by APS. Furthermore, the effect of APS on RAW264.7 macrophage demonstrated APS increase the level of cytokines including TNF-α, GM-CSF and the production of NO. NF-κB protein levels are increased in response to APS. Blocking NF-κB with specific inhibitor resulted in decreased levels of NO and TNF-α. The results suggested that APS possess potent immunomodulatory activity by stimulating macrophage and could be used as an immunotherapeutic adjuvant.

PMID:
21937031
DOI:
10.1016/j.cellimm.2011.07.011
[Indexed for MEDLINE]

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