Send to

Choose Destination
Cell Immunol. 2011;271(2):329-34. doi: 10.1016/j.cellimm.2011.07.011. Epub 2011 Aug 25.

Characterization of polysaccharide from Astragalus radix as the macrophage stimulator.

Author information

Department of Genetics and Biochemistry, School of Medicine, Zhejiang University, Hangzhou, People's Republic of China.


Astragalus polysaccharide (APS) was obtained by hot water extraction, alcohol precipitation, gel-permeation chromatography and ultrafiltration. Fluorescence material 2-aminoacridone (2-AMAC) labeled APS bind to macrophage in a time- dependent manner and the binding can be remarkably inhibited by APS. Furthermore, the effect of APS on RAW264.7 macrophage demonstrated APS increase the level of cytokines including TNF-α, GM-CSF and the production of NO. NF-κB protein levels are increased in response to APS. Blocking NF-κB with specific inhibitor resulted in decreased levels of NO and TNF-α. The results suggested that APS possess potent immunomodulatory activity by stimulating macrophage and could be used as an immunotherapeutic adjuvant.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center