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Nat Immunol. 2011 Sep 20;12(10):918-22. doi: 10.1038/ni.2117.

ChIP-Seq: technical considerations for obtaining high-quality data.

Author information

1
Laboratory of Molecular Immunology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA. benjamin.kidder@nih.gov

Abstract

Chromatin immunoprecipitation followed by next-generation sequencing analysis (ChIP-Seq) is a powerful method with which to investigate the genome-wide distribution of chromatin-binding proteins and histone modifications in any genome with a known sequence. The application of this technique to a variety of developmental and differentiation systems has provided global views of the cis-regulatory elements, transcription factor function and epigenetic processes involved in the control of gene transcription. Here we describe several technical aspects of the ChIP-Seq assay that diminish bias and background noise and allow the consistent generation of high-quality data.

PMID:
21934668
PMCID:
PMC3541830
DOI:
10.1038/ni.2117
[Indexed for MEDLINE]
Free PMC Article
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