Format

Send to

Choose Destination
PLoS One. 2011;6(9):e24119. doi: 10.1371/journal.pone.0024119. Epub 2011 Sep 13.

Cord blood CD4+ T cells respond to self heat shock protein 60 (HSP60).

Author information

1
Department of Pediatric Immunology, Wilhelmina Children's Hospital, Utrecht, The Netherlands. jaalber2@umcutrecht.nl

Abstract

BACKGROUND:

To prevent harmful autoimmunity most immune responses to self proteins are controlled by central and peripheral tolerance. T cells specific for a limited set of self-proteins such as human heat shock protein 60 (HSP60) may contribute to peripheral tolerance. It is not known whether HSP60-specific T cells are present at birth and thus may play a role in neonatal tolerance. We studied whether self-HSP60 reactive T cells are present in cord blood, and if so, what phenotype these cells have.

METHODOLOGY/PRINCIPAL FINDINGS:

Cord blood mononuclear cells (CBMC) of healthy, full term neonates (n = 21), were cultured with HSP60 and Tetanus Toxoid (TT) to study antigen specific proliferation, cytokine secretion and up-regulation of surface markers. The functional capacity of HSP60-induced T cells was determined with in vitro suppression assays. Stimulation of CBMC with HSP60 led to CD4(+) T cell proliferation and the production of various cytokines, most notably IL-10, Interferon-gamma, and IL-6. HSP60-induced T cells expressed FOXP3 and suppressed effector T cell responses in vitro.

CONCLUSION:

Self-reactive HSP60 specific T cells are already present at birth. Upon stimulation with self-HSP60 these cells proliferate, produce cytokines and express FOXP3. These cells function as suppressor cells in vitro and thus they may be involved in the regulation of neonatal immune responses.

PMID:
21931651
PMCID:
PMC3172234
DOI:
10.1371/journal.pone.0024119
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Public Library of Science Icon for PubMed Central
Loading ...
Support Center