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Appl Spectrosc. 2011 Sep;65(9):967-80. doi: 10.1366/11-06398.

Review of super-resolution fluorescence microscopy for biology.

Author information

1
Department of Chemistry, University of British Columbia, Vancouver, BC, V6T 1Z1 Canada.

Abstract

Several methodologies have been developed over the past several years for super-resolution fluorescence microscopy including saturated structured-illumination microscopy (SSIM), stimulated emission depletion microscopy (STED), photoactivated localization microscopy (PALM), fluorescence photoactivation localization microscopy (FPALM), and stochastic optical reconstruction microscopy (STORM). While they have shown great promise for biological research, these techniques all have individual strengths and weaknesses. This review will describe the basic principles for achieving super resolution, demonstrate some applications in biology, and provide an overview of technical considerations for implementing these methods.

PMID:
21929850
DOI:
10.1366/11-06398
[Indexed for MEDLINE]

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