Format

Send to

Choose Destination
Nat Chem Biol. 2011 Sep 18;7(11):763-5. doi: 10.1038/nchembio.659.

Metabolomics annotates ABHD3 as a physiologic regulator of medium-chain phospholipids.

Author information

1
The Scripps Research Institute, La Jolla, California, USA.

Abstract

All organisms, including humans, possess a huge number of uncharacterized enzymes. Here we describe a general cell-based screen for enzyme substrate discovery by untargeted metabolomics and its application to identify the protein α/β-hydrolase domain-containing 3 (ABHD3) as a lipase that selectively cleaves medium-chain and oxidatively truncated phospholipids. Abhd3(-/-) mice possess elevated myristoyl (C14)-phospholipids, including the bioactive lipid C14-lysophosphatidylcholine, confirming the physiological relevance of our substrate assignments.

PMID:
21926997
PMCID:
PMC3201731
DOI:
10.1038/nchembio.659
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center