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Mol Cell. 2011 Sep 16;43(6):962-72. doi: 10.1016/j.molcel.2011.08.008.

The DEAD-box protein Ded1 modulates translation by the formation and resolution of an eIF4F-mRNA complex.

Author information

1
Department of Molecular and Cellular Biology, University of Arizona, Tucson, AZ 85721-0206, USA.

Abstract

The translation, localization, and degradation of cytoplasmic mRNAs are controlled by the formation and rearrangement of their mRNPs. The conserved Ded1/DDX3 DEAD-box protein functions in an unknown manner to affect both translation initiation and repression. We demonstrate that Ded1 first functions by directly interacting with eIF4G to assemble a Ded1-mRNA-eIF4F complex, which accumulates in stress granules. After ATP hydrolysis by Ded1, the mRNP exits stress granules and completes translation initiation. Thus, Ded1 functions both as a repressor of translation, by assembling an mRNP stalled in translation initiation, and as an ATP-dependent activator of translation, by resolving the stalled mRNP. These results identify Ded1 as a translation initiation factor that assembles and remodels an intermediate complex in translation initiation.

PMID:
21925384
PMCID:
PMC3268518
DOI:
10.1016/j.molcel.2011.08.008
[Indexed for MEDLINE]
Free PMC Article

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