Format

Send to

Choose Destination
Trends Biotechnol. 2012 Jan;30(1):8-16. doi: 10.1016/j.tibtech.2011.08.002. Epub 2011 Sep 15.

Imaging proteins inside cells with fluorescent tags.

Author information

1
University of Maryland, College Park, Department of Entomology and Program in Cell and Molecular Biology, College Park, MD 20742, USA.

Abstract

Watching biological molecules provides clues to their function and regulation. Some of the most powerful methods of labeling proteins for imaging use genetically encoded fluorescent fusion tags. There are four standard genetic methods of covalently tagging a protein with a fluorescent probe for cellular imaging. These use (i) autofluorescent proteins, (ii) self-labeling enzymes, (iii) enzymes that catalyze the attachment of a probe to a target sequence, and (iv) biarsenical dyes that target tetracysteine motifs. Each of these techniques has advantages and disadvantages. In this review, we cover new developments in these methods and discuss practical considerations for their use in imaging proteins inside living cells.

PMID:
21924508
PMCID:
PMC3246539
DOI:
10.1016/j.tibtech.2011.08.002
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center