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Methods Cell Biol. 2011;104:219-35. doi: 10.1016/B978-0-12-374814-0.00013-6.

Single nucleotide polymorphism (SNP) panels for rapid positional cloning in zebrafish.

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1
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.

Abstract

Despite considerable genetic and genomic resources the positional cloning of forward mutations remains a slow and manually intensive task, typically using gel based genotyping and sequential rounds of mapping. We have used the latest genetic resources and genotyping technologies to develop two commercially available SNP panels of thousands of markers that can be used to speed up positional cloning.

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