Send to

Choose Destination
See comment in PubMed Commons below
J Microsc. 2012 Jan;245(1):63-71. doi: 10.1111/j.1365-2818.2011.03544.x. Epub 2011 Sep 15.

Effects of aberrations and specimen structure in conventional, confocal and two-photon fluorescence microscopy.

Author information

Department of Engineering Science, University of Oxford, Parks Road, Oxford, UK.


Specimen-induced aberrations cause a reduction in signal levels and resolution in fluorescence microscopy. Aberrations also affect the image contrast achieved by these microscopes. We model the effects of aberrations on the fluorescence signals acquired from different specimen structures, such as point-like, linear, planar and volume structures, when imaged by conventional, confocal and two-photon microscopes. From this we derive the image contrast obtained when observing combinations of such structures. We show that the effect of aberrations on the visibility of fine features depends upon the specimen morphology and that the contrast is less significantly affected in microscopes exhibiting optical sectioning. For example, we show that point objects become indistinguishable from background fluorescence in the presence of aberrations, particularly when imaged in a conventional fluorescence microscope. This demonstrates the significant advantage of using confocal or two-photon microscopes over conventional instruments when aberrations are present.

Free full text
PubMed Commons home

PubMed Commons


    Supplemental Content

    Full text links

    Icon for Wiley
    Loading ...
    Support Center